The Islamic Bookbinding Tradition. A Book Archaeological Study

The technique of Islamic bookbinding explores the development of the bookbinding tradition in the Islamic world. Based on an assessment of the collections in the University Library Leiden, the various sewing techniques, constructions and the application of covering materials are described in detail. A comparative analysis of the historic treatises on bookbinding provides further insight in the actual making of the Islamic book. Apart from that, it becomes clear that distinctive material characteristics can be indicative for production in a certain period or region. The general perception of Islamic bookbinding as a weak structure best typified as a case-binding is refuted by the findings. Instead, Karin Scheper demonstrates how diverse methods were used to create sound structures, which fundamentally changes our understanding of the Islamic bookbinding practice.Medieval and Early Modern Studie

IL-21 promotes the expansion of CD27+CD28+ tumor infiltrating lymphocytes with high cytotoxic potential and low collateral expansion of regulatory T cells

Contains fulltext : 118572.pdf (publisher's version ) (Open Access)BACKGROUND: Adoptive cell transfer of tumor infiltrating lymphocytes has shown clinical efficacy in the treatment of melanoma and is now also being explored in other tumor types. Generation of sufficient numbers of effector T cells requires extensive ex vivo expansion, often at the cost of T cell differentiation and potency. For the past 20 years, IL-2 has been the key cytokine applied in the expansion of TIL for ACT. However, the use of IL-2 has also led to collateral expansion of regulatory T cells (Tregs) and progressive T cell differentiation, factors known to limit in vivo persistence and activity of transferred TIL. The use of alternative T cell growth factors is therefore warranted. Here, we have compared the effects of IL-2, -15 and -21 cytokines on the expansion and activation of TIL from single-cell suspensions of non-small cell lung cancer, ovarian cancer and melanoma. METHODS: We applied the K562-based artificial APC (aAPC) platform for the direct and rapid expansion of tumor infiltrating lymphocytes isolated from primary cancer specimens. These aAPC were engineered to express the Fc-gamma receptor CD32 (for anti-CD3 antibody binding), the co-stimulatory molecule 4-1BBL, and to secrete either IL-2, IL-15 or IL-21 cytokine. RESULTS: Although IL-2 aAPC induced the greatest overall TIL expansion, IL-21 aAPC induced superior expansion of CD8+ T cells with a CD27+CD28+ "young" phenotype and superior functional cytotoxic effector characteristics, without collateral expansion of Tregs. CONCLUSION: Our data rationalize the clinical application of IL-21-secreting aAPC as a standardized cell-based platform in the expansion of "young" effector TIL for ACT

Defining the Critical Hurdles in Cancer Immunotherapy

ABSTRACT: Scientific discoveries that provide strong evidence of antitumor effects in preclinical models often encounter significant delays before being tested in patients with cancer. While some of these delays have a scientific basis, others do not. We need to do better. Innovative strategies need to move into early stage clinical trials as quickly as it is safe, and if successful, these therapies should efficiently obtain regulatory approval and widespread clinical application. In late 2009 and 2010 the Society for Immunotherapy of Cancer (SITC), convened an "Immunotherapy Summit" with representatives from immunotherapy organizations representing Europe, Japan, China and North America to discuss collaborations to improve development and delivery of cancer immunotherapy. One of the concepts raised by SITC and defined as critical by all parties was the need to identify hurdles that impede effective translation of cancer immunotherapy. With consensus on these hurdles, international working groups could be developed to make recommendations vetted by the participating organizations. These recommendations could then be considered by regulatory bodies, governmental and private funding agencies, pharmaceutical companies and academic institutions to facilitate changes necessary to accelerate clinical translation of novel immune-based cancer therapies. The critical hurdles identified by representatives of the collaborating organizations, now organized as the World Immunotherapy Council, are presented and discussed in this report. Some of the identified hurdles impede all investigators, others hinder investigators only in certain regions or institutions or are more relevant to specific types of immunotherapy or first-in-humans studies. Each of these hurdles can significantly delay clinical translation of promising advances in immunotherapy yet be overcome to improve outcomes of patients with cancer

Hands-on research : Its place in conservation training, in practice and the need for us to write about it.

Medieval and Early Modern Studie

Shifting sands of writing inks in Yemen. The occurrence of sparkling particles in Yemeni manuscripts

This article focuses on the analysis of glossy inks from a body of manuscripts from Yemen preserved in the University Library of Leiden, the phenomenon having been noted for the first time in Zabīd in the 2000s. Arab sources, including Yemeni, contain ink recipes in which the sparkling effect is sought after and obtained through the use of various ingredients. Laboratory analysis, meanwhile, reveals that the shiny effect results from particles (mica and sand, sometimes with a preparation based on red pigment) spread after the writing exercise. The use of these particles between functionality and aesthetics, which seems to be peculiar to Yemen, is at the center of the study and opens a new field of investigation.Medieval and Early Modern Studie

Omslag, bulletin van de Universiteitsbibliotheek Leiden en het Scaliger Instituut / 2003 - no 1

Vakpublicati

No evidence that polymorphisms of the vanishing white matter disease genes are risk factors in multiple sclerosis

Febrile infections are known to cause exacerbations in the white matter disorders 'vanishing white matter' (VWM) and multiple sclerosis (MS). We hypothesized that polymorphisms in EIF2B1-5, the genes involved in VWM, might be risk factors for the development of MS or temperature sensitivity in patients with MS. We found no difference in the frequencies of 15 EIF2B1-5 variants between patients with MS and healthy controls, and none of the variants showed significant deviation of the Hardy-Weinberg equilibrium. Furthermore, sequencing data of EIF2B1-5 in 20 patients with MS and measurement of the activity of eIF2B complex in patient-derived lymphoblasts did not support our hypothesis. © SAGE Publications 2008